The domains of rat liver sulfite oxidase. Proteolytic separation and characterization.
نویسندگان
چکیده
منابع مشابه
Electron paramagnetic resonance of the tungsten derivative of rat liver sulfite oxidase.
Sulfite oxidase purified from livers of tungsten-treated rats has been used for EPR studies of tungsten substituted at the molybdenum site of the enzyme in a fraction of the molecules. The EPR signal of W(V) in sulfite oxidase is quite similar to that of Mo(V) in its line shape and in its sensitivity to the presence of anions such as phosphate and fluoride. Hyperfine interaction with a dissocia...
متن کاملPurification and properties of sulfite oxidase from chicken liver. Presence of molybdenum in sulfite oxidase from diverse sources.
Sulfite oxidase (EC 1.8.3.1) has been purified from chicken liver by modification of a procedure previously applied to bovine liver. The enzyme appears homogeneous as judged by its behavior in sedimentation velocity experiments and polyacrylamide disc gel electrophoresis. A new activity stain for the enzyme on acrylamide gels is described. Despite its apparent homogeneity, the enzyme consistent...
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While the existence of a molybdenum cofactor common to nitrate reductase, xanthine oxidase, and sulfite oxidase has been finnly established by studies from several laboratories (1-6), the nature of the molecule has not been elucidated. Information available to date has shown that the molybdenum cofactor complex is noncovalently attached to the proteins (2, 4) and is probably a small, dialyzable...
متن کاملHepatic Sulfite Oxidase
Sulfite oxidase (EC 1.8.3.1), when reduced by sulfite, exhibited the electron paramagnetic resonance spectrum of molybdenum(V). Specific calorimetric analyses demonstrated that the purified enzyme contained 1 molybdenum per heme. The electron paramagnetic resonance signal of molybdenum, which was generated by the addition of sulfite, was enriched by the purification procedure to the same degree...
متن کاملMolecular Basis of Sulfite Oxidase Deficiency from the Structure of Sulfite Oxidase
The molybdenum-containing enzyme sulfite oxidase catalyzes the conversion of sulfite to sulfate, the terminal step in the oxidative degradation of cysteine and methionine. Deficiency of this enzyme in humans usually leads to major neurological abnormalities and early death. The crystal structure of chicken liver sulfite oxidase at 1.9 A resolution reveals that each monomer of the dimeric enzyme...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1978
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)34240-0